SQL Server Maintanance questions

Dear Friends,

I am planning to to turn the below items OFF to my production server Database.

-auto shrink

-auto create statistics

-auto update statistics

-auto close

Question 1: Do i need to restart SQL Agent or will this affect the GUI connected to this database

Qustion 2: Do i need to restart the services connected to this Database

Question 3 :What will happed to Database if i turn 'auto shrink OFF' to 'auto shrink ON'. I mean, Will database goen offline

Thanks in Advance

Parixit